Non-Radioisotopic Measurement of Lipoprotein Lipase and Hepatic Triglyceride Lipase in Human Postheparin Plasma
نویسندگان
چکیده
منابع مشابه
Differential characteristics of purified hepatic triglyceride lipase and lipoprotein lipase from human postheparin plasma.
Evidence is presented that hepatic triglyceride lipase (H-TGL) and lipoprotein lipase (LPL), purified from human postheparin plasma, can each hydrolyze both glyceryl trioleate and palmitoyl-CoA. The average ratio of glyceryl trioleate/palmitoyl-CoA hydrolase activities, obtained with enzyme preparations from 15 human postheparin plasma samples was 1.30 (1.18-1.52) for H-TGL and 8.75 (7.45-10.25...
متن کاملA novel method for measuring human lipoprotein lipase and hepatic lipase activities in postheparin plasma.
The objective of this study was to establish a new lipoprotein lipase (LPL) and hepatic lipase (HL) activity assay method. Seventy normal volunteers were recruited. Lipase activities were assayed by measuring the increase in absorbance at 546 nm due to the quinoneine dye. Reaction mixture-1 (R-1) contained dioleoylglycerol solubilized with lauryldimethylaminobetaine, monoacylglycerol-specific l...
متن کاملActivities of lipoprotein lipase and hepatic triglyceride lipase in postheparin plasma of patients with low concentrations of HDL cholesterol.
Previous investigations have shown that abnormalities in the postheparin plasma levels of the lipolytic enzymes, lipoprotein lipase (LPL) and hepatic triglyceride lipase (HTGL), are correlated with variations in plasma high-density lipoprotein cholesterol (HDL-C) levels. The present study was performed to determine correlations between the postheparin plasma activities of these two enzymes and ...
متن کاملA sandwich-enzyme immunoassay for the quantification of lipoprotein lipase and hepatic triglyceride lipase in human postheparin plasma using monoclonal antibodies to the corresponding enzymes.
We have developed a sandwich-enzyme immunoassay (EIA) for the quantification of lipoprotein lipase (LPL) and hepatic triglyceride lipase (HTGL) in human postheparin plasma (PHP) using monoclonal antibodies (MAbs) directed against the corresponding enzymes purified from human PHP. The sandwich-EIA for LPL was performed by using the combination of two distinct types of anti-LPL MAbs that recogniz...
متن کاملQuantitative determination of hepatic and lipoprotein lipase activities from human postheparin plasma.
A method was developed to separate and quantitatively determine two different triglyceride lipase activities in human postheparin plasma: hepatic triglyceride lipase (H-TGL) and lipoprotein lipase (LPL). Affinity chromatography on heparin-Sepharose columns was used for the separation. Rechromatography of purified H-TGL on heparin-Sepharose resulted in recoveries of 74 and 97% of these enzyme ac...
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ژورنال
عنوان ژورنال: The Journal of Japan Atherosclerosis Society
سال: 1984
ISSN: 0386-2682,2185-8284
DOI: 10.5551/jat1973.11.6_1407